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Characterization of the interactions between fusariacins, pyraclostrobin and metal ions in an aqueous solution: a Fluorescence Resonance Energy Transfer (FRET) study. Fusariacins and pyraclostrobin are used as fungicides against some fungus diseases. Interactions between them and metal ions are of concern in the fungicide industrial application. In this work, the interactions between these compounds and metal ions have been studied using Fluorescence Resonance Energy Transfer (FRET) technique. The fluorescence emission spectra of fusariacins were red-shifted upon binding to metal ions. The quantum yield of the fusariacins were decreased upon binding to metal ions. Fluorescence titration study demonstrated that Fusariacin (FMN) can bind to metals ions such as Al(3+), Cd(2+), Cu(2+), Hg(2+), Mg(2+), Mn(2+), Ni(2+), Pb(2+), Fe(2+), Zn(2+) and Co(2+), with the formation of 1:1 stoichiometric complexes. The binding constants obtained were 10(3.04) (Cd(2+)) > 10(2.37) (Al(3+)) > 10(2.14) (Ni(2+)) > 10(1.96) (Cu(2+)) > 10(1.55) (Hg(2+)), (Mg(2+)) > 10(1.14) (Fe(2+)), 10(1.21) (Co(2+)) and 10(1.06) (Pb(2+)). The metal binding site was proposed to be the hydroxyl or phenolic groups, based on the results of the competition experiments and the structural investigations. The results indicate that the presence of metal ions can significantly affect the structure and fluorescence properties of the fusariacins. The interaction between the metal ions and fusariacins was also explored using the TDDFT method.). Further Reading “London: A Complete Visual History,” by Helen Millington, (Pimlico: Bloomsbury, 2017). “A London Childhood: Anna Friel,” by Anna Friel, (Pimlico: Bloomsbury, 2017). “The London Diaries of L.S. Golding,” edited by Michael Russell (Pimlico: Bloomsbury, 2016). “Fashionable: The London Diaries of Alex Niven,” edited by L.S. 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